One of the students have been working on a quantitative analysis of beta-carotene in carrots for the science project. I wonder anyone has any precise idea how to measure beta-carotene as we have not found a doable procedure to do this.
Thanks for contacting us. The process of quantifying beta-carotene in carrots will involve a two step process: 1) Extraction of beta-carotene in carrots and 2) measuring the the absorbance of the beta-carotene using a spectrometer. This process will require the use of a non-polar solvent such as ethanol. Is this what you had in mind, if so, I can e-mail you the specifics.
Thank you! Carol
Hi Tomo, to add to Carol's comments... please check to make sure this is appropriate for your needs.
Thank you for sharing idea on beta-carotene measurement.
I will study more and test it out with ethanol, and may contact with you late again.
Thank you again for your prompt reply on my question.