thayamichi: Hi, One of the students have been working on a quantitative analys…

thayamichi's picture
thayamichi

Hi,
One of the students have been working on a quantitative analysis of beta-carotene in carrots for the science project. I wonder anyone has any precise idea how to measure beta-carotene as we have not found a doable procedure to do this.
Thank you

Oct 4 2017 - 12:40pm
Carol.Oshiro's picture
Carol.Oshiro

Hi Tomo!

Thanks for contacting us. The process of quantifying beta-carotene in carrots will involve a two step process: 1) Extraction of beta-carotene in carrots and 2) measuring the the absorbance of the beta-carotene using a spectrometer. This process will require the use of a non-polar solvent such as ethanol. Is this what you had in mind, if so, I can e-mail you the specifics.


Thank you! Carol

Oct 5 2017 - 2:25pm
sasakidy's picture
sasakidy

Hi Tomo, to add to Carol's comments... please check to make sure this is appropriate for your needs.

Oct 5 2017 - 2:48pm
thayamichi's picture
thayamichi

Hi Carol,

Thank you for sharing idea on beta-carotene measurement.

I will study more and test it out with ethanol, and may contact with you late again.

Thank you again for your prompt reply on my question.

Tomo,

3 hours ago